做luciferase需要注意些什么为取得最佳测定效果，在用单管的荧光测定仪测定时，样品和测定试剂混合后到测定...
To increase detection sensitivity and response dynamics, we genetically engineered firefly luciferase to detect specific intermolecular interactions through modulation of its luminescence activity. This concept has been applied in covalent, noncovalent, and allosteric design configur...
做luciferase需要注意些什么网友 1 最佳答案 回答者:网友 为取得最佳测定效果,在用单管的荧光测定仪测定时,样品和测定试剂混合后到测定前的时间应尽量控制在相同时间内,例如30秒内。由于温度对酶反应有影响,所以测定时样品和试剂均需达到室温后再进行测定。为保证荧光素酶检测试剂的稳定性,可以采取适当分装后避光保...