Wash the slides twice in wash buffer A for 2min under gentle agitation. Aspirate the wash solution after the last washing. 25. Calculate and add 40μL of ligase solution for each sample. Add 8μL of the 5× Amplification stock and 31.5μL of high-purity water, plus 0.5μL ofpolymerase...
After soni- cation, centrifugation, and pre-clearing, 1 mg of total cell lysate for each reaction was incubated at 4 1C overnight with 20 ml (dry volume) protein G-Sepharose beads (Amersham Biotech, Piscataway, NJ), which were conjugated with 1 mg experimental antibody or IgG antibody. ...
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Commercial cell lines were authenticated by microsatellite analysis using the PowerPlex kit (Promega Corporation, Madison, WI; last authentication: December 2015). The cells were checked for Mycoplasma spp. contamination by PCR every 3 weeks, and the contaminated cells were discharged. Dendritic cell ...
[1] 1. A continuous automated process for preparing an acid doped polybenzimidazole, PBI, polymer film membrane sheet for use in a fuel cell, the process comprising in the following sequence in automation, - providing a PBI film membrane sheet for processing; - in a washing stage, exposing ...
Cells were labeled with 1 µCi of [3H]-acetate (3600 mCi/mmol; Amersham International, Piscataway, NJ, USA) for 24 h. The synthesis of radiolabeled cholesterol, FPP [26], and IPP [17] were measured after lipid extraction, separation by thin-layer chromatography (TLC), and liquid scintil...
Nr6a1-1RNAs (Figure 7B). It should be mentioned that we have been struggling to achieve a pool of biotinylated oligonucleotides that specifically retrieves the lnc-Nr6a1-2isoform, but all attempts to solve this problem have so far been unsuccessful. After washing the complexes under denaturing ...
DRIVING POWER DEVICE FOR WASHING MACHINEPROBLEM TO BE SOLVED: To suppress the rotation of a rotary tank at the time of washing and to rotate the rotary tank at a high speed at the time of dehydration.KOO HYOUNG-MO具 亨謨LEE MIN-SOO...
we walked into the rooms of the old people, interacted with them and presented them with flowers to show our care and love. Subsequently, we did some cleaning and washing for them, followed by some performances, including singing songs, dancing and magic shows, which ...
据说剪纸的产生和宗教仪式有密切的关系, 很多剪纸作品反映了人们对于超自然力量 (supernatural power)的信仰。大家最熟悉不过的形象就是门神。 Since the Qing dynasty, peoplebegan to make paper cutting of gods and pasted them on doors, in order to d...