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this is ideal. The bottom firing speakers have reasonable volume, although the sound is tinny. You can also configure your PC's settings under "Sounds" to use your laptop's speakers and those on the Arzopa simultaneously to help flesh out the sound....
Thermo-life A12966 3PRIME FITC PAGE OD 10 ￥514.00 OD 10 Thermo-life A14676 PRIMER HPLC 11-59 BP OD 21-25 ￥514.00 OD 21-25 Thermo-life CK202020 PCR2.1 VECTOR 20 REACTIONS ￥514.00 20 REACTIONS Thermo-life CS30010 CSTPRO PLASMID MINI (10 PREP) 10 PREP ￥514.00 10 PREP ...
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Crosslinking was terminated by the addition of 1/10 volume 1.25 M glycine for 5 min at room temperature followed by cell lysis and sonication (Bioruptor, Diagenode), resulting in an average chromatin fragment size of 200 bp. Fragmented chromatin was then used for immunoprecipitation using...
A one-tenth volume of the sonicated lysate was saved for the input fraction control. The LWD1 or TCP22- associated chromatin complexes were immunoprecipitated by incubating the lysate with 3 ml rabbit polyclonal antisera generated with recombinant LWD1 (amino acid residues 1–73) or a polypeptide...
reporter gene driven by promoters containing TBS (mini35S was used as a negative control) (Student’sttest; *<0.005). Similar results were observed in three independent experiments. Full size image Electrophoresis mobility shift assay showed that TCP20 and TCP22 could form a protein–DNA complex...
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Types IV-A1 and IV-A3 ternary complexes were reconstituted by incubation of 10 µM type IV effector complexes and 15 µM target DNA for 10 min at RT in a total volume of 200 µl SEC buffer. Subsequently, Type IV-A1 assembly reactions were injected into a Superose 6 Increase 10/...