Contrary to permeabilization, the are not affected in any detectable way by the procedure, so that [alpha-32P]GTP and conversion to [alpha-32P]GDP can be studied over a period of time for the measurement of steady-state activity. The results show that careful control of electric field ...
Enzymatic synthesis of α- 32P and α- 33P labelled deoxyribonucleotides involves the transfer of radiolabelled phosphorus from either γ- 32P adenosine triphosphate ( γ-ATP) or γ- 32P guanosine triphosphate ( γ-GTP). Subsequent removal of these ribonucleotides is essential for the preparation...
摘要: Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Earth, Atmospheric, and Planetary Sciences, 1993.Includes bibliographical references (leaves 135-141).关键词: ALKYLATION ADP-RIBOSYLATION GTP-BINDING PROTEIN ISLET-ACTIVATING PROTEIN ...
【推荐2】Arf家族蛋白参与蛋白质的囊泡运输,它们有两种状态,结合GDP的不活跃状态和结合GTP的活跃状态。GTP和ATP的结构和性质相似,仅是碱基A被G替代。活跃状态的Arf蛋白参与货物蛋白的招募和分选,保证货物蛋白进入特定囊泡等待运输。下列相关叙述和推测正确的是( ) A.GTP是由鸟嘌呤、脱氧核糖和3个磷酸基团结合而成...
B.GTPC.UTPD.CTP14.生物体内最基本、最普遍的调节方式为:()A.细胞水平B.酶水平C.激素水平D.神经水平15.阻遏蛋白作用于乳糖操纵子的部位为:()A.结构基因B.终止基因C.操纵基因D.启动子简答1.\x05简述原核生物DNA复制的历程.2.\x05简述遗传密码的定义及其基本特点.3.\x05简述蛋白质生物合成的过程. 答案...
Guanine nucleotides (guanosine 5'-O-(3-thiotriphosphate) greater than GTP greater than guanosine 5'-O-(2-thiodiphosphate) greater than GMP) noncompetitively inhibited the binding of radioligand. Photolysis of [32P] BzATP-prelabeled membranes resulted in incorporation of radiolabel into a protein ...
Incubation of rat ovarian plasma membranes with [γ- 32P]guanosine 5′-triphosphate (GTP) in the presence of an adenosine triphosphate (ATP)-trapping system results in the labeling of a single protein, M r33000 ± 3000 designated 'a' (Amir-Zaltsman Y., Ezra E., Walker M., Lindner H.R...