A typical DSF reaction contained 30 µg of HIV Env, ±10 µg Fabs, 6 µL of the 50× dye (diluted in PBS from the supplied 5000x SYPRO orange stock in DMSO) and the volume was adjusted with PBS to 25 µL in a clear PCR tube. The temperature of the reaction ...
The top 25 differentially unmethylated regions for each cell type comprise a human cell-type-specific methylation atlas of 1,246 markers (Fig.3and Supplementary Table4). These regions are uniquely unmethylated in particular cell types (average methylation 13%) and methylated in all other samples ...
Transcripts with unexpected domain combinations, such as combinations of eukaryotic with typical bacterial domains, were also subjected to read coverage analysis. For that purpose, we mapped the raw reads of the novel taxa onto their respective Trinity assemblies, using Bowtie 2.1.0 with the non-...
Because oncolytic viruses circumvent typical drug-resistance mechanisms, they may represent an effective therapy for chemotherapy-resistant tumor initiating cells. A Nestin-targeted oncolytic herpes simplex virus efficiently replicated within and killed neuroblastoma tumor initiating cells preventing their ability...
According to our phylogenetic analysis, the H133Y variation in ST/MT of TSPyV resulted from recurrent fourth/back substitution (toggling) in the ORF1-encoded position, producing a typical signature of positive selection and suggesting its involvement in adaptation of TSPyV. In contrast, the other...
The genome contains the standard set of 37 genes usually present in animal mtDNAs and a large non-coding fragment putatively corresponding to the region (A+T-rich) responsible for the control of replication and transcription. All genes are arranged in the gene order typical of Pancrustacea. ...
This is mainly attributable to the tendency of BANNER to tag multiple terms that refer to the same entity; by giving precedence to a term that ends in “-ase(s)”, the typical effect is to select the full name of the enzyme ahead of its tagged abbreviation and/or EC number. For ...
Scientists have transfused lab-made red blood cells into a human volunteer in a world-first trial that experts say has major potential for people with hard-to-match blood types or conditions such as sickle cell (镰状细胞) disease. The research could someday mean an end to long searches for...
typical marker genes for identified cell types. Dot size represents the proportion of expressing cells and color indicates average expression level in each identified cell type.gSpatial distributions of selected marker genes show the number of transcripts captured by Stereo-seq.hThe spatial gene ...
The inheritance of the centrosome during human fertilization remains mysterious. Here we show that the sperm centrosome contains, in addition to the known typical barrel-shaped centriole (the proximal centriole, PC), a surrounding matrix (pericentriolar material, PCM), and an atypical centriole (di...