While much prior work has explored the constraints on protein sequence and evolution induced by physical protein-protein interactions, the sequence-level constraints emerging from non-binding functional interactions in metabolism remain unclear. To quantify how variation in the activity of one enzyme const...
a self-labeling protein (SLP) and a fluorescent protein. The SLP is selectively labeled with a fluorescent ligand that intramolecularly binds to the binding protein, resulting in a ‘closed’ conformation of the sensor. The
Abdelmajid KM, Ramos L, Leandro L et al (2012) The ‘PI 438489B’by ‘Hamilton’SNP-based genetic linkage map of soybean [Glycine max (L.) Merr.] identified quantitative trait loci that underlie seedling SDS resistance. J Plant Genome Sci 1(1):18–30 Article Google Scholar Abdelmajid...
An orange carotenoid protein (OCP) has been implicated as a blue-green light sensor that induces NPQ in cyanobacteria. Discovered in vitro, this process involves a light-induced transformation of the OCP from its dark, orange form (OCPo) to a red, active form, however, the mechanisms of ...
The recommended shelf life for this product is 1 year from date of receipt.Application Note: For IHC, epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections. 靶标信息 TET2 is a dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5...
Sortase A is a transpeptidase enzyme that requires an LPXTG recognition sequence on the N-terminal fragment and an oligoglycine sequence on the C-terminal fragment and catalyzes the formation of a peptide bond between the two fragments [25]. ...
Target Protein:ACACA IR:Immunogen Range:2307-2355/2458 Clonality:Polyclonal Isotype:IgG Entrez Gene:32 Swiss Prot:Q13085 Source:KLH conjugated synthetic peptide derived from human Acetyl Coenzyme A Carboxylase 1/2:2307-2355/2458 Purification:affinity purified by Protein A ...
ACADM protein is a medium chain specific (C4 to C12 straight chain) acyl Coenzyme A dehydrogenase. The enzyme catalyzes the initial step of the mitochondrial fatty acid beta oxidation pathway. ACADM expression is induced during periods of fasting, when reliance on fatty acids for energy is increas...
The reactions proceeded cleanly and completely (Supplementary Results, Supplementary Figs 1–3). Protein characterization confirmed generation of the intended folded protein cAbLys3-pCys104: peptide mapping using proteolytic digestion and liquid chromatography-mass spectrometry (LC-MS)/MS confirmed that ...
Natural proteins exhibit rich structural diversity based on the folds of an invariably linear chain. Macromolecular catenanes that cooperatively fold into a single domain do not belong to the current protein universe, and their design and synthesis open