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centrifugation for 1 min at 9000 × gat 4 °C. Nuclear extract lysis was done by adding 180 µl Buffer B + (10 mM HEPES, 10 mM KCL, 1 mM DTT, 400 mM NaCl, 1% IGEPAL, 1x cOmpleteTMEDTA free) and incubated for 15 min on a rotating wheel. To ...
The environmental samples were homogenized on a rotating wheel, pre-filtered using a 300-μm mesh to remove putative debris, and then filtered through a 5-μm TMTP filter (Millipore, Merck, France). The filters were stored at −80 °C until they were used. DNA preparation and ...
Question: A potter's wheel moves from rest to an angular speed of 0.14 rev/s in 32 s. What is the angular acceleration? Speed and Acceleration Speed is the rate of change of position with respect to time, and acceleration is the rate of change of ...
The big engine is heavier than the small engine, so the car uses extra energy every time it accelerates or drives up a hill. The pistons and other internal components are heavier, requiring more energy each time they go up and down in the cylinder. ...
PURPOSE: A power transmission device for an electric bicycle is provided to transfer the rotating force of a driving motor to wheels with a simple structure and to rotate a driving sprocket through a hollow rotating part without the rota... 안창덕 被引量: 0发表: 2011年 The sprocket as...
Accelerometers were installed on the axle box in most tests to investigate the mechanism or the effects of wheel polygonisation. Because the wheelset is a rotating component, its vibration feature may differ from that of the axle box, especially under high-speed conditions. Owing to gyroscopic ...
The water accelerates the propagation velocity of ultrasonic waves. However, the capability of ultrasonic waves to pass through the fractures slightly contributes to the presence of water inside the fractures and mainly depends on whether the migration of the coal matrix water covers the fracture area...
The aligned NF were collected by 12 mm circular glass coverslips (VWR, Bristol, CT, USA) attached on an aluminium rotating wheel, powered by a high-speed direct current (DC) motor. The electrospinning was conducted using the following parameters; an electrical potential of 20 kV, 1.0...
The cars are built to operate without a steering wheel, accelerator(油门) or brake pedal. "Our software and sensors do all the work," Urmson said. "The vehicles will be very basic—we want to learn from them and adapt them as quickly as possible—but they will take you where you want...