In order to enhance the process efficiency, bio-augmentation technology has been examined, where on the basis of composting method, screened and optimized bacteria with high-efficiency degradation are used to enhance the treatment effect while effectively shorten the treatment time. At present, a ...
Random primers Invitrogen Cat# 48190011 dNTPs Thermo Scientific Cat# R0193 Dithiothreitol Invitrogen Cat# Y00147 RNase OUT Invitrogen Cat# 10777019 SYBR Green Thermo Scientific Cat# K0253 Critical commercial assays Quick-DNA™ Fecal/Soil Microbe Kit Zymo Research Cat# D6012 PureLink™ RNA Mini ...
The engineering analysis of bioheat equation and penile hemodynamic relationships in the diagnosis of erectile dysfunction: part II-model optimization using the ANOVA and Taguchi method. Int J Impot Res. 2008;20:285–94. Article CAS PubMed Google Scholar Udelson D, Nehra A, Hatzichristou DG, ...
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The list of primers used for cloning is given in Table S1. The plasmids were introduced into either E. coli DH5α or Mach1 cells (Thermo Fisher Scientific) using heat-shock transformation and the sequence of the gene in the plasmid was shown to be correct by DNA sequencing of both ...
Sequences of all the primers are shown in Additional file 1: Table S3. The ex- pression level of each miRNA was measured using the 2-DeltaDeltaCt method. MTT assay K562 and HL60 cells were respectively plated at 1 × 104 per well. The cells were transfected with 100 nM miR- 99a ...
Mini Nutritional Assessment as a Useful Method of Predicting the Development of Pressure Ulcers in Elderly Inpatients Midori YatabeFumie TaguchiIzumi Ishida...Junichi Yatabe Journal of the American Geriatrics Society Sep 2013 Objectives To determine the usefulness of the Mini Nutritional Assessment (MNA)...
Oligonucleotide sequences for gRNAs and primers. Source data Source Data Fig. 1 Statistical source data. Source Data Fig. 2 Statistical source data. Source Data Fig. 3 Statistical source data. Source Data Fig. 4 Statistical source data. Source Data Fig. 5 Statistical source data. Source Data...
Primer sequences are listed in the Key Resources Table. PCR products were run on an agarose gel (Thermo Fisher Scientific) stained with ethidium bromide (Fisher Scientific), and purified using a QIAQuick Gel Extraction Kit (QIAGEN). Isothermal assembly was performed using 1 μL digested plasmid...
(B) Right panel represents the RT-PCR amplification with rep-primers from the c-DNA template prepared from the co-infiltrated samples, with actin amplification as the loading control. Left panel represents the semi-quantitative PCR of 21 cycles amplification of 1.6Kb band with respective actin ...