Briefly, NRK-49F cell lysates were prepared and incubated overnight at 4 °C with FITC-KP1 (10 µg), anti-TβR2 antibody (Santa Cruz), and protein A/G plus agarose (sc-2003; Santa Cruz). The immunocomplexes were blotted with antibodies against FITC and TβR2, respectively. To...
Cell pellets were suspended in a nuclear extraction buffer containing 50 mM Tris·HCl, pH 7.4, 150 mM NaCl, 1 mM EDTA, 0.5% TRITON®X-100 with proteinase/phosphatase inhibitors. Whole cell lysates (1 mg) were precleared with Protein A/G PLUS Agarose (Santa Cruz) and incubated...
Notably, the combination of a first-generation EGFR TKI plus dasatinib was evaluated in a phase II clinical trial in patients with EGFR-mutant lung cancer and acquired resistance to either gefitinib or erlotinib [46]. Moreover, a phase I/II clinical trial of osimertinib plus dasatinib in EGFR...
The first is that indigenous scholars, and those sensitive to the work that they are doing, are completely rewriting the textbook version that we all have gone through at one point or another—whether it was in high school or in college. The textbook begins on the east coast and moves West...
Suppose that the time devoted to writing the book could instead have been used by one of the authors to either (1) go to law school, (2) write a novel, or (3) start a profitable business.例如,写这本教材需要作者很多时间,而这些时间亦可以用于(1)攻读法律,(2)写一本小说,(3)经商。 ?
So my advice is this: if you are a new author considering the use of a prologue, seriously think about why you chose to include it. Does it add to the story or is it just enabling you to do less work? Another question to ask yourself is: is the prologue really the best place to...
PTPN14/Pez (H-67) antibody Santa Cruz sc-68384; RRID: AB_2268987 PTPN14/Pez antibody Laboratory of Dr. Yeesim Khew-Goodall N/A YAP (D8H1X) XP® rabbit mAb Cell signaling 14074; RRID: AB_2650491 p53 antibody (CM5) Leica (Novocastra) P53-CM5P; RRID: AB_563933 BrdU antibody Becton...
Chemiluminescence detection used ECL-plus reagent (Pierce). To ensure equal protein loading across gels, membranes were stripped (Restore western blot stripping buffer; Pierce) and re-probed with a loading control antibody. For dot blots, protein samples were spotted onto nitrocellulose membranes using...
Quantification of immunohistochemical staining was performed by Image pro plus. Micro-CT scanning Micro-CT analysis was performed to assess lung tumor growth because the micro-CT images clearly distinguished the lung tumors from the surrounding tissue even without any contrast agent, and the ...
Besides an obvious advantage in specific recognition of tumor cells, a gene circuit with an AND–NOT logic is more capable of driving a robust output than an AND circuit, as any constraint established by the NOT gate would become fully removed under the targeted condition (e.g., p53-...