coli BL21 CodonPlus (λDE3) cells (Stratagene) were transformed with expression vector and grown in LB broth containing 50 μg/ml kanamycin at 37 °C. Once the A600 of the culture reached 0.6, protein expression was induced by the addition of IPTG to a final concentration of 1 mM. The ...
For the purpose, MN arrays were dissolved in 15 mL of PBS (pH 7.4) and then small aliquots of the resultant suspension was diluted up to 100 times with distilled water before measuring the particle size using the NanoBrook Omni particle sizer (Brookhaven, New York, NY, USA). 2.12. Ex-...
health care facilities The Practical Nursing program givesstudents a solid foundation to pursue advancedcollege training." Eastern Suffolk, BOCESTechnical Center Brookhaven Technical Center 350 Martha Avenue Bellport, NY 11713-1569 Phone: (631) 286-6592 or Fax: (631) 286-6588 E-mail:...
3.2.2. Physical Characterizations of QUE–CHI Nanoplex The size, polydispersity index (PDI), and zeta potential of the QUE–CHI nanoplex were characterized in triplicates by dynamic light scattering microscopy (DLS) after 100 × dilutions using Brookhaven 90 Plus nanoparticle size analyzer (...