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To determine precisely where Ankle2 is localized, we performed live imaging of brains from animals carrying Ankle2-GFPR and a transgene that labels the endoplasmic reticulum (ER): da-Gal4>UAS-Sec61β-tdTomato (Summerville et al., 2016). In NBs (large cell in Figures 2C–2E), the Ankle...
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