Well, you’ll go “ApE” over A Plasmid Editor, a program as simple in use as it is in name. ApE is free to download and runs across all three major platforms: Windows, OS X, and Linux/Unix. Within the program, users can view circular or linear maps of their DNA sequences. ...
有人用过质粒编辑软件A Plasmid Editor吗？求中文使用教程
we sought to assemble three different plasmids with varying sizes ranging from 12.3 to 26.8 kb using seven DNA fragments (Supplementary Figs.6b–d). These plasmids included a 12.3 kb plasmid harboring an (R,R)-2,3-butanediol (BDO) pathway and agfpgene...
Download: Download full-size image Figure 2. Establishment of a PAM-flexible dual-mutation base editor, CABE-RY, in HEK293T cells (A) Schematic diagram for construction of four dual-mutation base editors, CABE-1, CABE-2, CABE-3, and CABE-4. (B and C) Comparison of the C-to-T (B...
Isopropyl beta-d-thiogalactoside (IPTG)-inducedE. coliOne Shot BL21 (DE3) containing the respective plasmid (Supplementary Table10) was cultured in LB medium at 37 °C. Protein expression was induced at an optical density at 600 nm (OD600) of 0.8, bacteria were collected after centrifugati...
Koetje EJ, Hajdo-Milasinovic A, Kiewiet R, Bron S, Tjalsma H (2003) A plasmid-borne rap-Phr system of Bacillus subtilis can mediate cell-density controlled production of extracellular proteases. Microbiology 149:19–28. https://doi.org/10.1099/mic.0.25737-0 Article CAS PubMed Google Schola...
Sample input files are located in the input directory of the installation folder. You can also download by clicking on this link:Sample_input_files.zip The above zip file contains four files, located inside a folder named 'input':plasmid_genomes.fasta Plasmid dataset - 471 prokaryotic genomes ...
Comstock MJ (Series Editor): Advances in Chemistry Series, vol 181 Chapter Google Scholar Durand H, Clanet M, Tiraby G: Genetic improvement of Trichoderma reesei for large-scale cellulase production. Enzyme Microb Tech. 1988, 10 (6): 341-346. 10.1016/0141-0229(88)90012-9. Article CAS ...
coli. Cells are co-transformed with a target plasmid (blue) and a base editor plasmid (purple). Base editor expression is induced with arabinose. After 16 hours, cells are harvested, and the target plasmid is analyzed by high-throughput sequencing. c, Base editing in E. coli of a ...
Liu, B., Dong, X., Cheng, H.et al.A split prime editor with untethered reverse transcriptase and circular RNA template.Nat Biotechnol40, 1388–1393 (2022). https://doi.org/10.1038/s41587-022-01255-9 Download citation Received23 June 2021 ...