coli without its native 23-residue signal peptide (see Supplementary Table 15), was obtained from Twist Bioscience and inserted into pET-29b(+) using the restriction sites NdeI/XhoI. Hans-LanM was overexpressed on a 2-l scale and purified using the established protocol for Mex-LanM50, with ...
(Bionics). The final sequence included the chicken β-actin promoter, a nuclear localization signal sequence at both the 5′ and the 3′ termini, and sequences encoding eGFP linked by a self-cleaving T2A peptide. Template DNAs encoding gRNAs were synthesized and cloned into a pTwist Amp ...
Osteoarthritis (OA), known as one of the most common types of aseptic inflammation of the musculoskeletal system, is characterized by defects of hyaline cartilage, synovial inflammation, subchondral bone loss, and tissue hypertrophy [1]. Its main clinical symptoms are chronic pain and whole-joint l...
Structure elucidation of inactive-state GPCRs still mostly relies on X-ray crystallography. The major goal of our work was to create a new GPCR tool that would provide receptor stability and additional soluble surface for crystallization. Towards this ai
A donor-π-acceptor (D-φ-A) system of oligo-(phenyleneethynylene)) (OPE) was twisted by clamping both ends of the OPE with a right-handed helical peptide (SSA8=OPE). The induced twist in OPE was in a right-handed way. SSA8=OPE showed a weaker HOMO--LUMO band in the absorption ...
products such asThe Face Oil,The Cream, andThe Rich Cream(which all contain the brand’s patented Trigger Factor Complex, a cell-renewing technology featuring a blend of natural amino acids, high-grade vitamins and peptides) before key make-up artist Lisa Butler took care of product ...
免疫原:KLHconjugatedsyntheticpeptidederivedfromhumanGROAlpha 保质期:详见说明书 标记物:详见说明书 包装规格:0.1ml/100μg0.2ml/200μg 抗原来源:Rabbit 适应物种:详见说明书 克隆性:否 标识物:详见说明书 形态:详见说明书 保存条件:详见说明书 是否进口:是 ...
(B) Similarly, viral-vectored vaccines (i.e., Ads) can also encode the transgene antigen sequence or display peptide antigen on the capsid exterior. These vectors allow for in vivo expression and antigen processing via MHC class I and class II. (C) Virus-like-particles (VLPs) or protein-...
a novel probe was developed that successfully prevented the interference of unspecific signal caused by albumin in the imaging of liver fibrosis in a mouse model. The in vivo findings, in agreement with the in vitro data, highlight the severity of albumin interference and validate the effectiveness...
The SAXS model suggests that this peptide might interact with the major groove of the double-stranded region of ITS1. In addition, interaction of the N-terminal repeats of Nop9 with the duplex region of the RNA includes residues at the junction between the a1 and a2 helices of repeats R1...