On biopsy, p16 immunostaining is used to adjudicate equivocal diagnoses on routine H&E tissue sections; a positive p16 immunostain supports a diagnosis of high-grade SIL.doi:10.1053/j.scrs.2017.04.002DarraghTeresa M.Seminars in Colon amp Rectal Surgery...
Immunostains were performed on FFPE tissue sections using anti-Nestin and anti-Sox2 antibodies. Results: Cytoplasmic Nestin was diffusely/weakly positive in NTK and PFT (1-2+), diffusely/strongly positive in subset of pNF (2+), majority of SW (3+) and MPNST (3+), expressed in a ...
(HSIL).Methods:p16INK4a and HPV16/18 DNA in thin-layer slides from 89 specimens were detected by immunohistochemistry and the insitu hybridization.Results:A positive correlation was found between the positive rate of p16INK4a and cervical disease progression,the positive rate of p16INK4a in ...
MSI CRCs were identified by MLH1 and PMS2 immunostain absence together with BRAF mutation. RNA was analyzed with Nanostring. RT-PCR validated the results. Results: Comparing MSI CRC to SSA and SSA-D, 68 miRs were differentially expressed. For SSA to CRC, 32 genes were up and 36 down. ...
Nuclear stain, with or without cytoplasmic reactivity, was considered positive and a percentage of positive nuclei was calculated. Samples were then divided in three categories according to the number of p16INK4a -positive atypical keratinocytes: negative (< 1% positive nuclei), moderate: less than...
p16INK4A is currently used as a 'positive' immunohistochemical marker for CIN, which is proposed to aid the identification of high-grade cervical lesions [9]. To evaluate the use of D2-40 in helping the diagnosis of CIN, we performed immunoreactivity of D2-40, compared to p16INK4A, on...
Various lysates were subjected to SDS PAGE followed by western blot with 10883-1-AP (P16-INK4A antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. IF Staining of MDCK using 10883-1-AP Immunofluorescent analysis of (4% PFA) fixed MDCK cells using P16-INK4A antib...
In tail skin, the Cdkn2a locus was hyperactivated in the SKOs from 4 weeks (p19/Arf) and 8 weeks (p16/Ink4a) onward, as detected by RT-qPCR (Figure 5a and Supplementary Table S1), RNA sequencing (Supplementary Table S1 in the study by Verbinnen et al [2020b]), and immunoblotting...
We evaluated the utility of a WT-1/AE1-AE3 dual color immunostain to distinguish metastatic melanoma from metastatic carcinoma and reactive mesothelial cells in pleural fluids. Design: Forty-four pleural effusions, including 11 melanomas, 11 breast carcinomas, 10 non-small cell lung carcinomas,...
Design: Formalin-fixed, paraffin-embedded tissue samples for non-neoplastic tissue (n=523 cases), primary neoplasms (n=498), and metastatic neoplasms (n=248) were subjected to immunostain for PAX-8. Results: Non-neoplastic tissue: PAX-8 was constantly noted in glomerular parietal epithelial ...