Cyclic dimeric guanosine monophosphate (c-di-GMP) serves as a bacterial second messenger that modulates various processes including biofilm formation, motility, and host-microbe symbiosis. Numerous studies have
OsSRF3 is degraded by the ubiquitination pathway, and further research can be conducted on the key E3 ligase involved in its degradation. In addition to being phosphorylated by OsBAK1, we also identified 12 other phosphorylation sites in OsSRF3 (Supplementary Fig. 16), indicating the presence ...
Emerging viral infections are among the greatest challenges in the public health sector in the twenty-first century. Among these, most of the viruses jump
Studies inEscherichia colishowed that once the Cas13/crRNA complex is bound to its target RNA, the HEPN-nuclease domains become active and are capable of cleaving not just the intended target, but also RNA molecules that are in the vicinity of the Cas13/RNA complex, resulting in the non-sp...
Single agent therapy with small molecule TKIs is effective in malignancies dependent on mutated constitutively activated RTKs [e.g. Kit/a-PDGFR in GIST (IM, Sunitinib), EGFR in NSCLC and GBM (Erlotinib)] and non-RTKs such as, Bcr-Abl in CML (IM, Dasatinib). However, chronic therapy wit...
However, focusing multiple targets or multipathway treatment is relatively difficult to pursue the exact mechanism of action of a drug in conventional experiments [8]. The new generation of drugs focus on targeting specific proteins that are expressed based on cancer type [9]. Understanding the ...
This is likely due to the flexibility of the 2G11 and CR2 binding sites in the DVD format [32]. The BeAb blocked the uptake of BoNT/A and cleavage of SNAP25 in neuronal cells, and recapitulated the potency of the combination of the two parental IgGs in a mouse-protection assay, ...
A.M.D. performed the generation of Huh7 and Hepa GPR108 KO cell lines, designed and produced chimeric capsids, and conducted cellular binding and entry assays, cell fractionation, western blots, and transduction assays in cell lines. S.P. generated H1 HeLa GPR108 KO and rescue cell lines,...
The tumor cell lysis EC50 was calculated in GraphPad Prism software using non-linear regression analysis for log (inhibitor) vs. response-variable slope. 2.10. In vivo studies All animal experiments were conducted in compliance with guidelines from Institutional Animal Care and Use Committee of the ...
While large-insert WGS can be conducted on the PacBio platform, the cost to achieve relatively deep coverage across the entire human genome for routine structural variation analysis is high. To take advantage of the PacBio long reads and reduce costs, we developed PacBio-LITS, a large-insert...