What is a limiting reagent? 02:12 How much minimum volume of CO at STP is needed to react completely wit... 03:48 How much of lime (CaO) can be obtained by the calcinations of 300 g of... 02:32 What volume of H(2) at STP is required to reduce 0.795 g of CuO to giv......
Blocking the import of nutrients essential for cancer cell proliferation represents a therapeutic opportunity, but it is unclear which transporters to target. Here we report a CRISPR interference/activation screening platform to systematically interrogate the contribution of nutrient transporters to support ca...
abasic site 脱碱基位点,无碱基位点 abaxial 远轴的 abequose 阿比可糖,beta脱氧岩藻糖 aberrant splicing 异常剪接 aberration 象差;畸变;失常 abiogenesis 自然发生论,无生源论 ablastin 抑殖素(抑制微生物细胞分裂或生殖的一种抗体) abnormal distrbution 非正态分布 abnormality 异常,失常;畸形,畸变 ABO blood ...
This may enable selection of more active CRISPRa populations, as only those cells which can activate the Puror gene under such limiting conditions will survive. If this hypothesis is correct, then it is possible that reducing the functionality of our SA sgRNA further, for example, by employing ...
Within the context of a cell, multiple targets compete for CaM, making free CaM a limiting reagent in the cytosol (89, 90). However, a number of CaM-binding proteins are found near or within the membrane that may serve as sinks. Among these proteins are ion channels including the NaV, ...
Essentially, any of the PCR reagents can be rate and performance limiting [22] including PCR protocol; and (4) competing reactions. The samples shall be tested for inhibition, which is easy done using RNA or DNA spikes [23], [24]. It can also be observed by performing a serial dilution...
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Scaling up production of dsRNA is a limiting factor for field applications; thus we established a bacterial expression system based on pET28-BL21(DE3) RNase III-. Its dsRNA expression is three-fold greater than that of the widely-used dsRNA expression system L4440-HT115 (DE3), which has inc...
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Subarachnoid hemorrhage (SAH) is a devastating neurological injury with high morbidity and mortality that is mainly caused by early brain injury (EBI). Progranulin (PGRN) is known to be involved in various biological functions, such as anti-inflammation and tissue repair. This study aimed to inves...