c, Quantification of INF-γ levels in the AD Neu/AC (+/−) iMGL and CD4+ or CD8+ T cells using ELISA assay (n=8 biological replicates; P-value from two-way ANOVA with Tukey multiple comparisons test). (a, c) In
) within the same cells using two-tailed, paired t tests (p < 0.0001 for pcDNA3.1, p = 0.0024 for UL12.5). N = 14 cells for pcDNA3.1, N = 13 cells for UL12.5, and data were pooled from three experiments. For panels B and D, data are reported as mean ±...
Tools/methods compared: Benjamini-Hochberg, Storey’s q-value, conditional local FDR (LFDR), FDR regression (FDRreg), independent hypothesis weighting (IHW), adaptive shrinkage (ASH), Boca and Leek’s FDR regression (BL), and adaptive p-value thresholding (AdaPT). Recommendation(s): Modern ...
On the overall, the feature elimination procedure highlighted compositional features that in most cases are confirmed as the most discriminative by statistical analysis (Alanine, Leucine, Asparagine, and Glutamine at the N-terminus, Threonine in the whole sequence) or that are supported by previous fi...
RNAlysis supports multiple approaches and statistical methods for enrichment analysis, including classic gene set enrichment analysis, permutation tests [20], background-free enrichment analysis [21, 22], and enrichment for ordinal or continuous variables. RNAlysis can automatically retrieve enrichment anal...
For example, if the value p < 0.05 is used, conducting twenty independent significance tests within a data set is likely to result in one comparison being significant just by chance. There are numerous multiple comparison procedures to adjust statistical analysis for type I error inflation, for ...
Notes We use the word “spot” to reference either the un-split paired biological read, or the single unpaired biological read. John Spouge, Statistical Computational Biology Group, National Library of Medicine, National Institutes of Health, Personal communication....
In the Additional file 1, in the section “Statistical test of CV(FPKM) groupings” we show that group 1 has a significantly smaller (at less than the .001 level) average CV(FPKM) than either group 2, 3 or 4 and that group 4 is significantly larger (at less than the .005 level)...
the statistical significance is calculated. The measure of statistical significance for an ORF is the expected number of ORFs in one megabase of random sequence at the same significance level or better, where the random sequence has the same statistics as the genome in the sense of a third orde...
Unless otherwise specified, all our statistical tests are guided by the following principles: (1) Since the random continuation rule varied the duration of each experimental session, we only report results from the first 50 (guaranteed) periods of the game. (2) In period 1, each individual is...