Cryogenic electron microscopy structural analysis revealed that the TdpA hexamer binds one strand of encircled duplex DNA via hydrogen bonds arranged in a spiral staircase conformation. Nevertheless, the TdpAB–DNA interaction was sensitive to the hydrophobicity of the PT sulfur. PTs inhibit ATP-driven...
BIO 7505: Biology: A Molecular Approach, Laboratory Microscope Theory and Operation I. What Microscopy Is: As your instructor lectures them, write-inwww.invitrogen.com)
The MB I class introduces first year graduate students to the most widely used approaches in biophysics and structural biology such as various spectroscopic methods and structure determination techniques such as NMR, X-ray crystallography, cryo-electron microscopy, fluorescence microscopy, and single ...
Cell morphology: SEM was employed in two studies [37,77], while light microscopy was used in one study [36]. Overall, changes in shape (from spindle to round), cell detachment, shrinkage, and membrane ruptures were observed. Cell cycle: All four studies [36,72,75,79] investigating cha...
Transmission electron microscopy (TEM), which allows a large population of particles to be observed with high lateral resolution; scanning electron microscopy (SEM), which determines topochemical data and surface defects; and a combination of both: transmission and scanning electron microscopy (STEM), ...
A postscript, courtesy of one of Banerjee’s Canadian Light Source collaborators, CLS Spectromicroscopy beamline scientist Jian Wang: “Also very interesting thatProf. Banerjee’s lastNature Communicationspaperusing CLS STXM and other techniques and computation was published on June 28 in 2011, exactly...
If you have questions, please reach out via comments on the blog, issues on Github or via email! Posted in Calcium Imaging, Data analysis, Imaging, machine learning, Network analysis, Neuronal activity | Tagged Calcium Imaging, Data analysis, deep learning, electrophysiology, Microscopy | 3 Comme...
While the relationship remains largely unexplored in Brazil using individual-level data, only a limited number of studies have addressed it.Investigating the short-term association between exposure to particulate matter (PM10) smaller than 10 micrometers and ozone (O3), and resulting cardiovascular and...
Maxillary sinus augmentation using an engineered porous hydroxyapatite: a clinical, histological, and transmission electron microscopy study in man. Porous hydroxyapatite (HA) is a calcium-phosphate-based material that is biocompatible, nonimmunological, and osteoconductive, and has a macroporosity of a....
Examples included giving the context or “discovery story” for unknown pigments in order to solidify ideas learnt in microscopy; developing an object-based course through collaboration between a museum and university; or using actual data sets from X-ray fluorescence spectroscopy of a painting to ...