Extensive impact of non-antibiotic drugs on human gut bacteria. Nature 555, 623–628 (2018). Article CAS Google Scholar Atarashi, K. et al. Treg induction by a rationally selected mixture of Clostridia strains from the human microbiota. Nature 500, 232–236 (2013). Article CAS Google ...
The course was open to both graduate and upper-level undergraduate students at a large, public, research-intensive institution in the Mid-Atlantic region of the United States. The course met once per week for two and a half hours throughout the spring semester. The content goals of the cours...
Antibiotic treatment kills a large portion of a population, while a small, tolerant subpopulation survives. Tolerant bacteria disrupt antibiotic efficacy and increase the likelihood that a population gains antibiotic resistance, a growing health concern.
aeruginosa human infection biology. Included in these genes of unknown function is PA1414 (locus tag in P. aeruginosa strain PAO1), whose expression level in humans was 222-fold higher than that in the laboratory, and its expression level is the highest of the P. aeruginosa genes ...
The first step in microbiome research is to understand the advantages and limitations of specific HTS methods. These methods are primarily used for three types of analysis: microbe-, DNA-, and mRNA-level analyses (Fig.1A). The appropriate method(s) should be selected based on sample types and...
, molecular biology, structural biology, and the high throughput “omics” techniques have made possible an increasingly detailed understanding of the complex systems and pathways that control the fundamental cellular responses as well as how the molecular interactions occur at the atomic level. ...
The short-term feeding (2–3 days) of relatively high levels (6–15 mg/kg/day) of the ionophore polyether antibiotic, lasalocid, was reported to be an effective treatment for acute cryptosporidiosis in young calves [144], [145], [146] but was highly toxic when fed (at 8 mg/kg/day)...
CRISPR/Cas9-mediated re-sensitization of antibiotic-resistant Escherichia coli harboring extended-spectrum Beta-lactamases. J Microbiol Biotechnol. 2015;26:394–401. Article CAS Google Scholar Kamens J. The Addgene repository: an international nonprofit plasmid and data resource. Nucleic Acids Res. ...
Such a limitation has become increasingly apparent in TB, where numerous experimental studies have shown that AMR-associated genetic variants often reflect network-level metabolic adaptations to antibiotic-induced selection pressures (Supplementary Fig. 1, Supplementary Table 1)8,9,10,11,12. These ...
Then the antibiotic resistance cassette was eliminated using the FLP recombinase expressing plasmid pCP20. Thermo-sensitive pCP20 was cured from the resulting strains by two passages in LB in the absence of ampicillin at 42 °C. Double mutants were constructed following the same protocol but ...