Diamond Knife on a Leica EM UC7—ultramicrotome, and 50 nm thickness sections were obtained and mounted on EM-copper grids with formvar/carbon coating. Sections were post-stained in 4% UAc in water and Reynolds’ lead citrate for 5 min each, and then processed for imaging using a FEI...
Ultrathin (70 nm) sections were obtained using a diamond knife (ultra-grade, Diatome) and a Leica EM UC7 ultramicrotome and collected onto formvar-coated grids (Electron Microscopy Sciences). Thin sections were stained with uranyl acetate and Reynold’s lead citrate and examined in a JEOL J...
Blocks of resin embedded Borrelia were sectioned on a Leica UC7 ultramicrotome. For tomography, 150nm thick sections were collected onto the grids containing 15nm fiducial gold markers. Post-staining The grids were post-stained for 5 min with 2% uranyl acetate in 50% methanol and washed with ...
Ultra-thin sections with a thickness of 60 nm were generated using a Leica UC7 ultramicrotome (Leica Microsystems, Inc.) and contrasted with 2% uranyl acetate and Reinolds׳ lead citrate. Sections were imaged using a Hitachi H-7650 transmission electron microscope (Hitachi-High Technologies) at...
In this webinar the sample preparation workflow including the Ultramicrotome Leica EM UC7, its cryo-chamber Leica EM FC7 and the pre-preparation system Leica EM TXP will be given. The main part of this webinar will cover tips and tricks to reveal the...
Ultrathin sections of 70 nm were prepared with an ultramicrotome UC7 (Leica, Germany) using a DiATOME diamond knife (Switzerland) and were placed on 75 mesh pioloform carbon-coated nickel grids. The grids were stained with 1% uranyl acetate for 30 min and grids were examined in a Tec...
Thin sections were cut with a Leica Ultramicrotome Ultracut UC70 sections (60 nm), stained with uranyl acetate and lead citrate. Images were recorded with a Tecnai Spirit transmission electron microscope at 120 kV with bottom-mounted EAGLE 4Kx4...
95% and 100% ethanol for 5 min each and washed three times with distilled water. The film was mounted in rubber mold with Epon resin and incubated at 65 °C for 48 h. Resin blocks were cut into 70 nm thickness by a diamond knife on ultramicrotome (Leica EM UC7). Cell slices were ...
Polymerized resins were then sectioned at 90 nm thickness using a Leica EM UC7 ultramicrotome and imaged at 70 kV using a JEOL 1400 TEM equipped with an AMT CMOS imaging system. Mitochondrial structures (Aspect Ratio, Perimeter, Circularity) were analyzed and quantified by ImageJ. Form ...
(w/v) paraformaldehyde in phosphate-buffered saline (PBS) at 4°C overnight. Fixed leaves were embedded in LR White resin (Sigma-Aldrich) after dehydration through a graded alcohol series. Sections were prepared on a Leica Microsystem UC7 ultramicrotome. Ultrathin sections of ~70 nm were ...