To test this feature, we used Keio knockouts of non-essential genes (rmf or yejL) to reconstruct a point mutation in neighboring, essential genes, fabA or yejM (Table 1). In each case, the wild-type non-essential gene was properly delivered while the desired mutation in the essential ...
Monoamine oxidase A (MaoA) is an X-linked gene well known to influence behaviour: MaoA mice knockouts show increased aggressiveness57. An X-X* difference in expression of MaoA (or another X-linked gene) could therefore explain the differences observed in terms of attack latency. Further ...
By comparison with Gal3, knockouts (KOs) of two other galectins, Gal8 and galectin-9 (Gal9), shown to play a role in mTOR inactivation and AMPK activation leading up to autophagy (Jia et al., 2018) did not affect lysosomal status during damage for 30 min, or recovery from damage (...
B.B. Fredholm, J.F. Chen, S.A. Masino, J.M. Vaugeois Actions of adenosine at its receptors in the CNS: insights from knockouts and drugs Annu. Rev. Pharmacol. Toxicol., 45 (2005), pp. 385-412 CrossrefView in ScopusGoogle Scholar [23] N. Bertheleme, S. Singh, S.J. Dowell,...
different allelic combinations of genes, creating, at its most efficient, homozygous gene knockouts. Additionally, multiple copies of functional genes can be targeted all at once. This is especially relevant in polyploid plants such asCamelina sativawhich contain complex genomes with multiple chromosome ...
controls, although gray matter showed small overlap between autistic females and sexually dimorphic structures in controls. This overlap was more pronounced in white matter. Cerebral volume is perhaps not the best measure to assess similarities between sexually dimorphic and autism-specific structural ...
controls, although gray matter showed small overlap between autistic females and sexually dimorphic structures in controls. This overlap was more pronounced in white matter. Cerebral volume is perhaps not the best measure to assess similarities between sexually dimorphic and autism-specific structural ...
Evidence for an Extracellular Zinc-Veneer in Rodent Brains from Experiments with Zn-Ionophores and ZnT3 Knockouts Ionic zinc is found at a high concentration in some glutamatergic vesicles of the mammalian brain. Ionic zinc is also found chelated to macromolecules in t... I Nydegger,SM Rumschik...
Saam JR, Gordon JI: Inducible gene knockouts in the small intestinal and colonic epithelium. J Biol Chem. 1999, 274: 38071-38082. 10.1074/jbc.274.53.38071. Article CAS PubMed Google Scholar Soriano P: Generalized lacZ expression with the ROSA26 Cre reporter strain. Nat Genet. 1999, 22: ...
The most commonly used approaches try to modify individual reaction fluxes to redirect metabolic flux or to find a combination of gene knockouts leading to overproduction of the desired metabolite [28–31]. A first metabolite-centric approach for the analysis of genome-scale metabolic models was ...