A hydrophobic column (Hypersil GOLD™ aQ C18 Polar Endcapped HPLC Column, 100 mm × 2.1 mm, 1.9 μm) and a hydrophilic (Accucore™ 150 Amide HILIC HPLC Column, 100 mm × 2.1 mm, 2.5 μm) column were used for reverse phase (C18 column) and hydrophilic ...
Chromatographic separation was achieved on a Hypersil GOLD column (50×2.1mm I.D., 5μm) using a mobile phase consisting of acetonitrile–0.1% formic acid solution (30:70, v / v ) at a flow rate of 0.2mLmin 1 . Detection was performed on a triple quadrupole tandem mass spectrometer ...
Chromatographic separation was performed on a Hypersil GOLD aQ column (2.1 × 100 mm, 1.9 μm, Thermo Fisher Scientific, USA) with mobile phase A consisting of 0.1% formic acid in water and mobile phase B consisting of 0.1% formic acid in acetonitrile. The column temperature was ...
The column used was a Hypersil Gold C18 (2.1 × 100 mm, 1.9 μm), the column temperature is set at 40 °C. The injection volume is 5 μL. The mobile phase A is composed of 0.1% formic acid aqueous solution, and the mobile phase B is an acetonitrile solution. ...
Fast chromatographic separation was achieved on a Hypersil Gold column (100 × 2.1 mm i.d., 5 µm). Mass spectrometric detection was achieved using a triple-quadrupole mass spectrometer equipped with an electrospray ionization interface operating in positive ionization mode. Quantification...
Metabolome analysis was finished based on the liquid chromatography-mass spectrometry method. The liquid chromatography-mass spectrometry platform was based on Ultimate 3000LC (Thermo, Q Exactive) equipped with a Hypersil GOLD™ C18 Column (100 mm × 2.1 mm, 1.9 µm). The detailed ...
A Hypersil Gold HPLC column (Thermo Fisher Scientific; 100 × 2.1 mm, 1.9 μm) with a 16 min linear gradient and 0.2 mL/min flow rate was employed. Eluent A (0.1 % FA in water) and eluent B (MeOH) were used for positive polarity mode. Eluent A (5 mM ammonium acetate, pH 9.0)...
and reconstituting it,the team also spiked each sample with N-benzoyl-D5-glycine as an internal standard (tR = 4.27 min,m/z185.0969).They analyzed samples via liquid chromatography and mass spectrometry (LC-MS) using an Accela UHPLC system and aHypersil GOLD aQ column(both Thermo Scientific...
To analyze the products of different enzyme-digested peptidoglycan, an untargeted HPLC-MS/MS analysis was performed at the Central Library of Sothern Medical University. Ten microliters of peptidoglycan digests prepared as described above were separated on a Hypersil GOLD C18 column (1.9 μm, ...
We performed tryptic digest and MS as previously described14,16. Briefly, the separation of tryptic peptides prior to mass spectrometry was performed on a Hypersil Gold UHPLC column (1.9 µm, 50 × 1.0 mm) using an Accela 1250 UHPLC system (both Thermo Fisher Scientific, USA...