Adeno-associated virus (AAV) vectors are the leading platform for gene delivery for the treatment of a variety of human diseases. Recent advances in developing clinically desirable AAV capsids, optimizing genome designs and harnessing revolutionary biote
“Historically we’ve thought of bacteria as only having harmful behaviors, like infecting us and causing disease, but more recently scientists have discovered the microbiome, a rather magical bacterial community that lives in and on us and that keeps us alive,” Bassler said. “What my lab has...
Engineering microbial hosts for production of bacterial natural products. Nat. Prod. Rep. 33, 963–987 (2016). Article CAS PubMed PubMed Central Google Scholar Krivoruchko, A., Zhang, Y., Siewers, V., Chen, Y. & Nielsen, J. Microbial acetyl-CoA metabolism and metabolic engineering. ...
33 Although VLPs represent a promising platform for future development, VLPs derived from bacterial and insect cells can be contaminated with endotoxin or baculovirus, they lack mammalian glycosylation, and there are occasionally issues with formulation stability, precipitation and aggregation, or scale-up...
Further another advantage of photocatalytic ozonation is that it could be used for disinfection of municipal wastewater as many studies have been reporting bacterial inactivation [113,114,115] and reported no post-treatment regrowth of E. coli in the dark after 24 and 48 h. However, some studies...
For example, Mongolian indigenous cattle have evolved to survive during tremendously cold winters and have reduced susceptibility to parasites, bacterial and viral infections [9, 10]. In recent decades, efforts have been made to improve the genetic potential of local cattle through crossbreeding with ...
We only included the WindowMasker file for alignments to the bacterial database (-window_masker_db). For each contig, we counted the percentage of base pairs covered by alignments to each database. If more than 10% of a contig aligned to the rDNA database, we deemed it to be a ...
(transcripts per million reads of genes in each MAG) indicated. The tree was constructed with the anvi’o Bacteria_71 set of single-copy genes for both bacterial and archaeal MAGs and midpoint-rooted.CLevel of taxonomic novelty of the MAGs. The number of classified and unclassified genomes ...
Current methods that rely on presenting cell adhesion molecules (CAMs) on bacterial surfaces are limited by the lack of a generalizable strategy to identify such molecules targeting bacterial membrane proteins in their natural states. Here, we introduce a whole-cell screening platform designed to ...
a,bT-DNA constructs for (a) GUS and (b) CRISPR/Cas9 (Cas9 and aPDS-targeted sgRNA) expression. The GUS construct consists of a GUSPlus gene interrupted by a cas1 intron (in), to prevent bacterial expression, under the control of a CaMV 35S promoter, and a kanamycin resistance gene (...