品牌:Invitrogen 所属分类:培养基 货品编号: thermo.A-21206 品牌:thermo 品名:Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 规格:1mg 产品描述: To minimize cross-reactivity, these donkey anti-rab
Samples were run on NuPAGE 4–12% gel (Invitrogen, NP0322BOX) and transferred onto polyvinylidene fluoride transfer membrane. Primary antibodies used included anti-GABPA (1:4,000, Proteintech), anti-HA (1:1,000, CST) and anti-β-actin (1:5,000, CST, #4967). Secondary antibodies used ...
Secondary antibodies were used as follows and incubated for 45 min at RT: donkey anti-goat/rabbit Alexa Fluor 555 (Invitrogen, A-21432/A-31572, 1/500), donkey anti-rabbit/rat/goat Alexa Fluor 488 (Invitrogen, A-21206/A-21208/A-11055, 1/500) and DAPI (Sigma-Aldrich, D9542, 1/...
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A-21206, Invitrogen, Thermo Fisher Scientific, RRID:AB_2535792) and Alexa Fluor 555 (donkey anti-Alexa Fluor™ 555, 1:500, Catalogue No. A-31570, Invitrogen, Thermo Fisher Scientific, RRID:AB_2536180). Finally, tissue sections were again washed three times for 10 min each with PBS, ...
Briefly, cell lines were incubated with 500 ng/ml Hoechst (H1399, Invitrogen) for 10 min to label the nuclei. Subsequently, the cytoplasmic area was defined by a 5-pixel (~1.1 μm) annular ring from the nuclear region. Tracking of each cell was conducted using a Munkres ...
After three washes, the samples were incubated with secondary antibodies specific to anti-donkey rabbit IgG Alexa Fluor 488 (#A-21206, Invitrogen, MA, United States) or goat anti mouse IgG Alexa Fluor 555 (#A-21424, Invitrogen, MA, United States) at room temperature for 2 h. After ...
Subsequently, secondary antibodies, including C yTM5-conjugated donkey anti-goat (1:500, Jackson, 70575147), CyTM3-conjugated donkey anti-mouse (1:500, Jackson, 715165150) and Alexa Fluor-488-conjugated donkey anti-rabbit (1:500, Invitrogen, A-21206), were applied for 60 min. ...
Donkey anti-Rabbit IgG (H+L) Alexa Fluor 488 Invitrogen Cat# A-21206; RRID:AB_2535792 Donkey anti-Mouse IgG (H+L) Alexa Fluor 488 Invitrogen Cat# A-21202; RRID:AB_141607 Donkey anti-Mouse IgG (H+L) Alexa Fluor 555 Invitrogen Cat# A-31570; RRID:AB_2536180 Donkey anti-Rabbit IgG ...
Antibodies against the following molecules were used for immunofluorescence or Western blotting: Alexa Fluor 488 donkey α-rabbit IgG (H+L; A-21206, Invitrogen, 1:500), actin (20–33) (A5060, Sigma, 1:1000) and peroxidase-conjugated rabbit immunoglobulins (P0217, Dako, 1:5000). ...