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Peripheral neurons in mouse intestinal cryosections were labeled with rabbit anti-synapsin I antibody (Product # A-6442) and detected using Alexa Fluor® 488 goat anti-rabbit IgG antibody (Product # A-11008). This tissue was counterstained with DAPI (Product # D1306, D3571, D21490). View...
Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody (A-11008) in ICC/IF Immunofluorescence analysis of Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody Alexa Fluor® 488 conjugate was performed using HeLa cells stained with alpha Tubulin Rabbit Polyclonal Antibody (Product # PA5-...
Alexa Fluor 488 goat anti-rabbit IgG Thermo Fisher Scientific A-11008 (1:1000) Alexa Fluor 647 donkey anti-goat IgG Thermo Fisher Scientific A-21447 (1:1000) V5 Thermo Fisher Scientific R960-25 (1:1000, chromatin immunoprecipitation) Alexa Fluor 488 SSEA4 Thermo Fisher Scientific 53-8843-42...
(1:500 dilution, catalog no. A-11008, Thermo Fisher Scientific), goat anti-rabbit IgG (H+L) Alexa Fluor 555 (1:500 dilution, catalog no. A-21428, Thermo Fisher Scientific), goat anti-rat IgG (H+L) Alexa Fluor 555 (1:500 dilution, catalog no. A-21434, Thermo Fisher Scientific),...
THERMO KING 30150* THERMO KING 2925B06H5* THERMO KING 2020B40H1* TORO 5977* TRANSICOLD 50620192* TRANSICOLD 50010010* TRANSICOLD AC60148* TRANSICOLD 207892* TYC 22331* TYC 13C123* TYC 20113* TYC 3R4178* TYC 3600270* TYC 3600370* TYC BEL02* TYC AM5081* TYC BEL0* TYC HAP158* TYC ...
After washes with PBS containing 0.1% NP-40, the following secondary antibodies were used at room temperature: Alexa Fluor 488 anti-rabbit (Thermo Fisher Scientific; catalog no.: A-11008, 1:500 dilution) and Alexa Fluor 594 antimouse (Thermo Fisher Scientific: catalog no.: A-11005, 1:500...
Alexa Flour-488 secondary anti-rabbit (ThermoFisher, cat. no. A-11008, RRID:AB_143165, 1:1,000 dilution) was incubated along with DAPI. Images were collected using a Cytation5 automated microscope (Biotek) using a ×4 objective and cleaved caspase-3 intensity was quantified using onboard ...
(goat anti-rabbit Alexa 488, A-11008; ThermoFisher Scientific) for 1 h at 37 °C. After three washes in PBS, DNA was counterstained with DAPI in Vectashield mounting medium (Vector Laboratories). Nuclei were visualized using a Zeiss Axio Imager Z.1 epifluorescence microscope (Carl ...
Slices were re-sectioned to 40 µm (Leica CM 3050S) and then incubated with anti-bNOS (Rabbit polyclonal, cat #N7280, Sigma-Aldrich) followed by a fluorescent tagged secondary (goat anti-rabbit, alexa fluor 488 nm, A-11008, ThermoFisher) and anti-GFAP (Chicken polyclonal, ab4674...