Almost all radiolabellings of antibodies with 89Zr currently employ the hexadentate chelator desferrioxamine (DFO). However, DFO can lead to unwanted uptak
The phenotype of human decidual stromal cells (hDSC) and phagocytic function of rat bone-marrow-derived macrophages (rMac) was somewhat affected by radiolabelling.Our study demonstrates that [ 89 Zr]Zr-oxine and [ 89 Zr]Zr-DFO-NCS are equally effective in cell labelling. However, [ 89 Zr...
et al. 89Zr-DFO-J591 for immunoPET of prostate-specific membrane antigen expression in vivo. J. Nucl. Med. 51, 1293–1300 (2010). Article CAS PubMed Google Scholar Holland, J.P. & Lewis Jason, S. Zirconium-89 chemistry in the design of novel radiotracers for immuno-PET. in ...
Binding to cell surface GPC3 by unconjugated, DFO- and DOTA-conjugated αGPC3 was confirmed by flow cytometry (Fig.1). Normalized geometric mean fluorescence for αGPC3-DFO and αGPC3-DOTA were significantly elevated compared to control. These validated conjugates were subsequently radiolabeled with89...
rays, which are summarized inTable S2. DFO titration was used to determine ESA—the activity per total amount of metals—as per Holland,et al.[23]. To minimize non-specific binding of other metals by the DFO, the DFO was challenged by 50 mM DTPA. The titration was carried out by thin...
Scheme 5. Schematic depiction of biomolecule-DFO-conjugation via reaction of amino functionalities of the biomolecule with a DFO-active ester. OO N OH 5NH OH H NN 5 OO O N OH OOO FeCl3 OH FF FF EDC pyridine, RT, 24 h pH 6.5-7.0, pH 5.8-6.0, NaOH, RT, 16 h RT, 10 min RT,...
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Abstract The development of molecular imaging agents used for imaging of cancer tissue is of great importance for the early detection of cancer. Positron emission tomography (PET) radiopharmaceuticals consists of a positron-emitting radionuclide and a molecularstructure.89Zr-labeled monoclonal antibodies (...
Measuring the pharmacodynamic effects of a novel Hsp90 inhibitor on HER2/neu expression in mice using Zr-DFO-trastuzumab. PLoS One 2010, 5, e8859. 40. Dijkers, E.C.; Kosterink, J.G.; Rademaker, A.P.; Perk, L.R.; van Dongen, G.A.; Bart, J.; de Jong, J.R.; de Vries, E...
The labeling efficiency of 89Zr-DFO was determined while using radio-ITLC-SG with 100 mM DTPA as the mobile phase. The radio-ITLC plates were scanned using an instant imager scanner (Bioscan, DC, USA). Radioactivity measurements were performed in an ionization chamber (CRC-25PET; Capintec) ...