氧化损伤化学探针合成工艺传感器氧化应激过程产生的大量活性氧类物质会对细胞内蛋白或DNA等生物大分子造成损伤.8-oxodGuo是一种主要的DNA氧化损伤产物.DNA复制时,这种损伤常常会导致G→T的突变.因为8-oxodGuo不仅能与碱基C配对,还可以与碱基A配对.如果该损伤不能及时被修复,则会导致基因不稳定,从而产生基因或遗传...
ID-UPLC-MS/MS大鼠尿液8-oxodGuo目的建立一种快速,高效的同位素稀释超高效液相-串联质谱法(ID-UPLC-MS/MS),用于检测大鼠尿液中DNA氧化产物8-羟基脱氧鸟苷(8-oxodGuo).方法大鼠尿液经10 mmol/L的醋酸铵处理,加入同位素内标[^(15)N_(5)]8-oxodGuo,置于37℃生化培养箱孵育,离心后以5 mmol/L的醋酸铵(含...
DNA damage (comet assay) and 8-oxodGuo (HPLC-EC) in relation to oxidative stress in the freshwater bivalve Unio tumidus. Biomarkers 10, 41-57.S. Lemiere,C. Cossu-Leguille,A-M. Charissou,P. Vasseur.DNA damage (comet assay) and 8-oxodGuo (HPLC-EC) in relation to oxidative stress ...
Our structural analysis shows that hOGG1 can recognize and flip 8-oxodGuo even within NCPs; however, the interaction between 8-oxodGuo and hOGG1 in a NCP context is weaker than in free DNA due to competition for nucleosomal DNA by the histones. Binding of OGG1 and the flipping of 8-...
Takai D,Park SH,Takada Y,Ichinose S,Kitagawa M,Akashi M.UV-irradiation induces oxidative damage to mitochondrial DNA primarily through hydrogen peroxide:analysis of 8-oxodGuo by HPLC.Free Radical Research.Takai D,Park SH,Takada Y,Ichinose S,Kitagawa M,Akashi M.UV-irradiation induces oxidative ...