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Cells were collected by centrifugation onto glass slides, fixed at 4°C for 30 seconds by a buffered formalin aceton mixture (pH 6.6; 20 mg Na2HPO4, 100 mg KH2PO4, 30 mL H20, 45 mL aceton, and 25 mL formalin). Cells were incubated for 30 minutes at 37°C in the reaction medium ...
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