6. Load appropriate volumes of samples onto SDS-PAGE gel (loading quantity of protein sample depends on the concentration of extracted proteins). NOTE:At the same time, please load the pre-stained molecular weight markers to determine molecular weights and verify electrotransfer. ...
These events can be slowed down tremendously if samples are kept on ice or at 4°C at all times and appropriate inhibitors are added fresh to the lysis buffer. EDTA chelated calcium and magnesium ions. Sodium orthovanadate is an inhibitor of all tyrosine protein phosphatases. It must be ...