500,000 STAT2 KO HEK-293T cells were transfected with 1 μg of either an empty vector or a vector coding for GFP, STAT2-GFP, or NS5-FLAG. 24 h post transfection, cells were lysed in 300 µL of 1X RIPA buffer (Millipore, 20-188) supplemented with cOmplete, Mini Protease ...