1.细菌或原核生物16S rRNA: 细菌核糖体RNA(rRNA)有三种类型:5S rRNA(120bp)、16S rRNA(约1540bp)和23S rRNA(约2900bp)。5S rRNA基因序列较短,包含的遗传信息较少,不适于细菌种类的分析鉴定;23S rRNA基因的序列太长,且其碱基的突变率较高,不适于鉴定亲缘关系较远的细菌种类;16S rRNA普遍存在于原核细胞中,...
All individual sequences deposited in the 5S rRNA Database can be retrieved using the WWW-based, taxonomic browser at http://rose.man.poznan.pl/5SData/5SRNA.html++ + or http://www.chemie. fu-berlin.de/fb_chemie/agerdmann/5S_rRNA.html . The files with complete sets of data as well ...
Tandemly arranged repetitive regions (repeats) that encode 5S rRNA (5S rDNA) are an indispensable component of eukaryotic genomes. Typically, 5S rDNA repeats within a genome are very similar due to the concerted nature of the evolution of this type of repeats. Each 5S rDNA repeat consists of ...
The complete genomes also provide a growing set of data for comparative analysis of ribosomal proteins. In most ribosomes, the mass of the rRNA is significantly larger than that of the proteins. The protein–RNA interactions are extensive and the protein–protein interactions are more limited. ...
Genomic region encoding 5S rRNA (5S rDNA) is a popular tool for investigation of the molecular evolution and phylogeny of closely related animal and plant taxa. In this article, we present the results of the analysis of 5S rDNA polymorphism in two breeding races, Rakhiv and Hoverla, of the...
Bioinformatic analysis The contigs of IGS clones were assembled in BioEdit Sequence Alignment Editor 7.0.9.0 (Hall, 1999) and blasted against the GenBank database. The rRNA coding sequences were identified by pairwise comparisons using dot plot graphical outputs (http://www.vivo.colostate.edu/...
5S rRNA is an indispensable component of cytoplasmic ribosomes in all species. The functions of 5S rRNA and the reasons for its evolutionary preservation as an independent molecule remain unclear. Here we used ribosome engineering to investigate whether
S1 mapping showed that at least a significant portion of the 5S rRNA and tRNAArg(ACG) is co-transcribed in canola chloroplast, making trnR the last gene transcribed in an operon of which the final sequence is 5′-16S–tRNAIle–tRNAAla–23S–4.5S–5S–tRNAArg-3′. Various RNA termini rep...
The gene families under study were subjected to BLASTN search at the NCBI database; all coding regions (5S rRNA, 18S rRNA, 5.8S rRNA and U2 snRNA) showed high degrees of identity with others obtained from a wide variety of fish species. Spacer regions did not present any significant homolo...
Loop C of 5S rRNA is within a short distance from the loop of H84 of the 23S rRNA, whereas loop D closely approaches the H39 loop and the base of H42 (Fig. 1e). Integration of cp5S rRNA into any of these 23S rRNA sites would require very short (0–3 nt long) RNA tethers. ...