The GST fusion proteins were subsequently eluted from the lysate using Glutathione Sepharose 4B (GE Healthcare, Uppsala, Sweden) and solubilized in SDS loading buffer (containing 10% SDS, 50% glycerol, 0.05% bromophenol blue, 0.5 M Tris-HCl, pH 6.8 and 100 mM DTT). Then, the pellet was ...