Monoclonal antibodyELISAMaize kernelsMaize is one of the most important staple food crops worldwide. 5-Formyltetrahydrofolate (5-F-THF), the most dominant and stable folate derivative in maize kernels, is important to human health and acts key roles in several metabolic pathways and biological ...
Model simulations also provide evidence that 5-methylTHF (and not 5fTHF) is the predominant physiological binder/inhibitor of SHMT. Finally, the model simulations indicate that the 5fTHF futile cycle dampens the stochastic noise in FOCM that results from both folate deficiency and a common ...
5-formyltetrahydrofolate (5-formylTHF) is administered in these treatments, either as a rescue agent or as an enhancer. The only enzyme known to use 5-formylTHF as a substrate is 5,10-methenyltetrahydrofolate synthetase (MTHFS). This enzyme catalyzes the conversion of 5-formylTHF to 5,10...
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Pharmacokinetic analysis of (6S)-5-formyltetrahydrofolate (1-CF), (6R)-5-formyltetrahydrofolate (d-CF) and 5-methyltetrahydrofolate (5-CH3-THF) in patients receiving constant iv infusion of high-dose (6R,S)-5-formyltetrahydrofolate (leuc... Straw JA, Newman EM (1988) Pharmacokinetic anal...
The analytical recovery for 5-F-THF in the maize kernels with icELISA was 66-111%, and that with liquid chromatography-mass spectrometry (LC-MS) was 57-114%, indicating a good consistency between these two methods (Slope, 0.92; R 2, 0.98). We conclude that the sensitive and versatile ...
Preparations of radiolabeled 5-methyl-THF and 5-formyl-THF are described which resulted in enzymically active diastereoisomers, mostly in yields of 25–30%. The radiochemical purity of the preparations was close to 99%.doi:10.1016/0003-2697(71)90121-7P.F....
Intriguingly, only supplementation with 5-CHO-THF, as opposed to folic acid, offered significant and consistent protection against UVB-inflicted oxidative damage in the folate-deficient larvae. We concluded that the intrinsic folate status correlates with the vulnerability to UVB-induced damage in ...
methylene THF reductase-(NADPH)胺氧化还原酶类叶酸叶醛酸四氢叶酸盐类Methylenetetrahydrofolate reductase (MTHFR) catalyzes the synthesis of 5-methyltetrahydrofolic acid (5-CH(3)-H(4) folic acid), the methyl donor for the formation of methionine from homocysteine. A common C677T transition in the...