Promega Corporation, Madison, WI, USA). First-strand cDNA was synthesized in a 10 μL reaction volume containing 0.5 μg of RNA, 0.5 μL of PrimeScript RT enzyme mix I, 0.5 μL of Oligo dT primer (50 μM), 2 μL of random hexamers (...
For RT-qPCR analysis, after total RNA was extracted, the RNA was immediately reverse-transcribed into cDNA using the PrimeScript™ RT reagent Kit (Perfect Real Time) (Takara, cat# RR037A). RT-qPCR was subsequently performed using SYBR Green Real-time PCR Master Mix (Takara, cat# RR036A...
Isolated RNA was treated with RNase-free DNase I (TaKaRa, Tokyo, Japan) to eliminate contaminated DNA, and cDNA synthesis using 1 mg of total RNA was performed with the PrimeScript RT reagent kit (Perfect Real Time, TaKaRa) according to the manufacturer’s instructions. Real-time PCR was ...