Then, the two amplicons were inserted into the pTOPO-Blunt Simple vector (Aidlab Biotechnologies Co., Ltd.) using the ClonExpressTM II One Step Cloning Kit (Vazyme Biotech Co., Ltd.), respectively. A cry1Ac gene from Bacillus thuringiensis (Bt-cry1Ac, GenBank accession no. GU322939.1) was...