Here, the basic protocol of RNA-BisSeq, its current applications and limitations are described.doi:10.1016/bs.mie.2015.03.007Matthias Schaefermethods in enzymologySchaefer,M. (2015) RNA 5-methylcytosine analysis by bisulfite sequencing. Methods Enzymol., 560, 297-329....
5Sino-Danish College, University of Chinese Academy of Sciences, Beijing 100049, China; 6National Institute of Biological Sciences, Beijing 102206, China 5-methylcytosine (m5C) is a post-transcriptional RNA modification identified in both stable and highly abundant tRNAs and rRNAs, and in mRNAs. ...
(C) is converted to uracil and will be read as T in the sequencing. 5-methylcytosine (5mC) remains the same and 5-hydroxymethylcytosine (5hmC) forms cytosine 5-methylenesulfonate (CMS). The bisulfite modified DNA is further treated with specific APOBEC deaminase, which converts 5mC to ...
Although 5-methylcytosine (m5C) is a widespread modification in RNAs, its regulation and biological role in pathological conditions (such as cancer) remain unknown. Here, we provide the single-nucleotide resolution landscape of messenger RNA m5C modifica
5-hydroxymethylcytosine (5hmC), as a sixth DNA base with functions in transcription regulation, has been detected to be abundant in human and mouse brains and embryonic stem (ES) cells. In mammals, it can be generated by the oxidation of 5mC, a reaction mediated by the ten-eleven ...
The high level of 5-hydroxymethylcytosine (5hmC) present in neuronal genomes suggests that mechanisms interpreting 5hmC in the CNS may differ from those present in embryonic stem cells. Here, we present quantitative, genome-wide analysis of 5hmC, 5-methylcytosine (5mC), and gene expression in...
Integration of tanscriptome-wide RNA bisulphite sequencing (BisSeq), RNA-sequencing (RNA-seq) and RNA Immunoprecipitation (RIP)-seq analysis revealed that ALYREF specifically binds to hypermethylated m(5)C site in RAB, member RAS oncogene family like 6 (RABL6) and thymidine kinase 1 (TK1) ...
5-methylcytosine (m5C) is a post-transcriptional RNA modification identified in both stable and highly abundant tRNAs and rRNAs, and in mRNAs. However, its regulatory role in mRNA metabolism is still largely unknown. Here, we reveal that m5C modification