Mice were termly anesthetized and intraperitoneal injected with D-Luciferin (150 mg per kg body weight, 10 min prior to imaging) to monitor metastasis by in vivo imaging with the IVIS Spectrum Imaging System (Caliper Life Sciences, MA, USA). Only organs revealing signal of metastasis were ...
Images were obtained after 5 min by intraperitoneal injection of 100 μL (25 mg/mL) of D-Luciferin. Seven days later, the same experimental procedure was performed to obtain images again. (D) Kaplan-Meier plots of overall survival of mice with high circSOBP levels and controls. (E) HE ...
For bioluminescence imaging (BLI), D-luciferin (YEASEN, Shanghai, China) was injected into anesthetized mice and bioluminescence images were captured (Xenogen IVIS, PerkinElmer, Waltham, MA, USA). At week 12, mice were euthanized and applied to further histological analysis. All measurements were ...
Luciferin-luciferase assay mixture (100 μl) was added to 100 μl of cell lysates or 50 μl of extracellular material in 96-well microtiter plates and light emission was monitored in a 1250 LKB-Wallac luminometer. Results are expressed in bioluminescence relative light units (RLU) and ATP ...
After 3 h, cells were lysed, added to luciferin reagent (Promega, E1501), and luciferase activity was determined by measuring luminescence over 10 s with a Biotek Synergy H1 plate reader using Gen5 software. Data were normalized to untreated controls, plotted using GraphPad Prism, and analyzed ...
D-Luciferin,Eagle BioSciences,ecmbiosciences,eenzyme research,EMC microcollections,enzymeresearch,finescience,genesee scientific,gibco公司,gibco培养基,gibco胎牛血清,goldbio,golden west biologicals,Haematologic Technologies,Haematologic Technologies Inc,Hyglos,IBL,immunodx,Immunostep,ImmunoVision,innovagen,iris-biotech...
人肺动脉平滑肌细胞HPASMCD-荧光素钾盐,D-虫荧光素钾盐质量规格:>98%,BRD-Luciferin potassium RWPE2细胞,人上皮细胞兔晶体上皮细胞永生系,N/N1003A(RLE)细胞小鼠心肌细胞MCM多尼培南质量规格:>99%,BR,一水合物Doripenem LLC-WRC 256(大鼠腹水癌细胞) 5×106cells/瓶×2盐酸多沙普仑质量规格:>99%,USP31,...
品牌:solarbio | 货号:K004611P 英文名称Anti-CDKN2D Polyclonal Antibody 宿主Rabbit 别名INK4D;p19;p19-INK4D 应用WB IF 稀释比例WB 1:500-2000. IF 1:50-100. 交叉反应Human 蛋白分子量18kDa Gene ID1032 保存Store at -20°C. Avoid freeze / thaw cycles. ...
The systemic distribution of cancer cells in different organs of the nude mice was assessed through bioluminescence imaging after intraperitoneal injection of D-luciferin (150 mg/kg). Beginning 7 days post-injection, the mice were examined for brain metastases weekly via bioluminescence imaging. ...
After 48 h of transfection, tobacco leaves were infiltrated with 1 mM luciferin (Gold Biotechnology, 115144-35-9). Luciferase bioluminescence images were taken with the Chemi-Image System (Tanon 5200Multi, China). For co-immunoprecipitation in rice protoplasts, the proteins were extracted from...