View all Troubleshooting I have a qPCR master mix from another vendor that has ROX™ passive reference dye in a separate tube. How much should I add for my reactions in the QuantStudio™ 3 and 5 Real-Time PCR Systems? Are my run fi...
Each experiment was performed at least 3 times in triplicate. Mesenchymal-like MeT5A cells treated with MS-275 (250 nM) for 72 hours. C RT-qPCR showing the expression of β1 and α5 Integrin subunits from total RNA of mesenchymal-like MeT5A cells treated with MS-275 (250 nM) ...
View all 3.3 Usage Allyl Mercaptan is a synthetic flavoring agent that is a stable, color-less liquid of garlic-like odor. it should be stored in glass or tin con-tainers. it is used in artificial garlic flavors for application in condiments at 3 ppm, and in baked goods at 2 ppm. ...
Based on the results of qPCR analysis, FXYD5 siRNA #1 at 10 nM concentration was applied for functional assays. Where indicated, the siRNA treatment was performed as described above for the second time after two days from the first silencing. Collection of cell secretomes and lysates The ...
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The cDNA was diluted 50 times with double distilled water to provide the template for the RT-qPCR analysis. Twenty μL reaction volumes containing 10 μL SYBR premix ExTaq (TAKARA, Dalian, China), 0.6 μL of each of the forward and reverse primers, 2 μL cDNA template, and 6.8 μL ...
"With up to 6GB RAM and 128GB ROM, Mi MIX 2S unleashes the full potential of the Qualcomm Snapdragon 845 for amazing performance," the company said in a statement. Dubbed the "Camera Beast", Redmi Note 5 "is a photography powerhouse that takes amazing photos anywhere, at any time", tha...
cDNA was synthesized from total RNA using Maxima First Strand cDNA Synthesis Kit for RT-qPCR (ThermoFisher Scientific, Waltham, MA, USA). The RT-PCR was performed on individual cDNAs by using SYBR® Green PCR master mix in the CFX384 Touch Real-Time PCR System. The primer sequences are...
Ferroptosis is known to play a crucial role in diabetic osteopathy. However, key genes and molecular mechanisms remain largely unclear. This study aimed to identify a crucial ferroptosis-related differentially expressed gene (FR-DEG) in diabetic osteopat
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