Longer incubation times (up to 1 h) did not affect the patterns or intensities of the bands, indicating that the system had reached thermodynamic equilibrium within 15 min. Samples were then loaded on a native 1% agarose gels in 0.5× TBE buffer. Electrophoresis was performed for 90 min at ...
Then the samples were mixed with 6× native RNA loading dye [1× TBE, 12% glycerol, 0.085% xylene cyanol, 0.085% BPB] and electrophoresed in 12% non-denaturing polyacrylamide gel under 1× TB buffer. The gels were stained by SYBR™ Gold Nucleic Acid Gel Stain (Invitrogen), and ...
Genomic DNA was digested with Hind III, electrophoresed on a 0.8% agarose gel in 0.5× TBE buffer, and then transferred to a nitrocellulose membrane. For hybridization a 492 bp PCR-amplified gusA gene product was labeled using the PCR DIG Probe Synthesis kit (Roche, ...
The reactions were incubated at room temperature for 20 min, and then 2 μl of 5× TBE loading buffer was added to each reaction. The samples were loaded onto a 6% DNA retardation gel (Invitrogen) and run at 300 V for 15 min. The gel was then dried and exposed to x-ray film. ...
Beads were washed three times with wash buffer (50 mM Tris-HCl pH 7.5, 250 mM NaCl, 0.4 mM EDTA, 0.1% NP-40, 1 mM DTT, 0.4 U/µl RNasin (Promega)). Samples were separated on Novex® 4%-20% TBE gel (Thermo) and stained with Coomassie brilliant blue. The protein-containing ...
The harvested cells were resuspended in the binding buffer (25 mM Tris-HCl pH 7.5, 500 mM NaCl, 3 mM β-mercaptoethanol, 5 mM imidazole, and 1% glycerol). Following sonication and centrifugation, the lysate was loaded onto Ni-NTA resin. The resin was washed three times with the...
Genomic DNA was digested with Hind III, electrophoresed on a 0.8% agarose gel in 0.5× TBE buffer, and then transferred to a nitrocellulose membrane. For hybridization a 492 bp PCR-amplified gusA gene product was labeled using the PCR DIG Probe Synthesis kit (Roche, http://www.roche....
static rt_uint8_t rx_buffer[RT_SERIAL_RB_BUFSZ + 1]; pms_device_t dev = parameter; while (1) { rt_memset(&msg, 0, sizeof(msg)); result = rt_mq_recv(&pms_mq, &msg, sizeof(msg), RT_WAITING_FOREVER); if (result == RT_EOK) ...
The protein:DNA complexes were resolved by electrophoresis through 5% nondenaturing polyacrylamide gels in 0.25 X TBE buffer. Electrophoresis was conducted for 2 1/2 hours at 150 V. The gels were then vacuum dried and exposed to X-ray film overnight with an intensifying screen screen at -80°...
(19:1) polyacrylamide gels. The gels were run at 110 V for 30 min in 0.5× TBE (Tris/borate) buffer made from a 10× TBE stock solution. Then, the gel was stained by GelRed staining, and the images were processed by ImageJ software41. All shifted bands, including the upper ...