RT-qPCR检测先关mRNA水平变化 Total RNA was isolated from cells using TRIzol reagent (TAKARA Biotechnology, Japan), and total mRNA (1 μg) was reverse transcribed to cDNA using PrimeScript RT Master Mix (TAKARA Biotechnology, Japan). Transcript expression was assessed by subjecting synthesized cDNA...
RT-PCR, quantitative PCR (qPCR), siNRAs Total RNA was isolated following the standard method from cell lysis in Trizol reagent (RNAiso Plus, Takara), and cDNA was synthesized using 5×Primescript® RT Master Mix (Takara), according to the manufacturer’s instructions. RT-PCR was performed ...
Total RNA was isolated with TRIzol Reagent (Invitrogen), and cDNA was synthesized using PrimeScript™ RT Master Mix (Takara, China). The real-time PCR (RT-PCR) analysis was performed with SYBR Advantage qPCR Premix (Clontech, USA). Primer sequences are listed in the Supplemental Table S1....
RNA was reverse transcribed using PrimeScript RT Reagent Kit (Takara) and analysed by RT–qPCR in triplicates using the Maxima SYBR Green/ROX qPCR Master Mix (Thermo Fisher Scientific). The qPCR reactions were run on the QuantStudio 7 Real-Time PCR system (Thermo Fisher Scientific). GAPDH was...
Reagents were purchased from TAKARA (5 × PrimeScript RT Master Mix, RR036A, SYBR® Premix Ex Taq™ II, RR820A). GAPDH was chosen as an internal quantitative control. All the primers were purchased from Invitrogen and the primer sequences were shown as Additional file 1: Table S...
mRNA was reversely transcribed into complementary DNA (cDNA) in each experimental and control group using the PrimeScript RT Master Mix Kit (Takara Bio Inc., Dalian, China). Then, cDNA was tested by PCR via the SYBR Premix Ex Taq Kit (RR420a; Takara, Tokyo, Japan). To normalize the ...
Total RNA (1 μg) extracted from pericarp of the genotypes M82, yft1, YFT1-CDS, and YFT1-RNAi tomatoes, at 35 dpa, 47 dpa and 54 dpa, was used to generate templates to synthesize first chain cDNA using the PrimeScript™ RT Master Mix kit (TAKARA, Dalian, China), with three biolo...
Total RNA was extracted with Trizol Reagent (Thermo Fisher) and reverse transcription of miRNA and mRNA performed with the SYBR PrimeScript miRNA RT-PCR Kit and PrimeScript RT Master Mix (Takara, Dalian, China) according to the manufacturers’ instructions. MiRNA levels were determined by SYBR Gree...
Next, 200–500 ng of RNA was reverse-transcribed with PrimeScript RT Master Mix (TAKARA) and after that quantified by RT-qPCR using the QuantiFast SYBR Green RT-PCR Kit (Qiagen) and a CFX96 Touch Real-Time PCR Detection System (Bio-Rad). Each cycle included 10 s at 95 °C ...
Total RNA was extracted with Trizol Reagent (Thermo Fisher) and reverse transcription of miRNA and mRNA performed with the SYBR PrimeScript miRNA RT-PCR Kit and PrimeScript RT Master Mix (Takara, Dalian, China) according to the manufacturers' instructions. MiRNA levels were determined by SYBR ...