PCR amplification was performed using the TransStart FastPfu FLY DNA Polymerase (AP231, civic bioscience) following instructions of the manufacturer with primers described in supplemental Table 4. Sanger sequencing of amplicons was performed on an ABI 3730xl DNA Analyzer (Applied Biosystems) at McGill...
The PCR reaction system was constituted of 4 μL of 5×TransStart FastPfu buffer, 2 μL of 2.5 mM dNTPs, 0.8 μL of forward primer (5 μM), 0.8 μL of reverse primer (5 μM), 0.4 μL of TransStart FastPfu DNA polymerase, 10 ng of template DNA, and ddH2O to make up a final...