1× Phosphate buffer saline (PBS) Meilunbio Cat #: MA0015,Lot#: MA0015-Dec-03E14 Dithiothreitol (DTT) Sangon Cat #: DB0058, Lot#: F222BA0006 Sodium chloride Sinopharm Cat #: 10019318, Lot#: 20200915 TransStart Fastpfu DNA Polymerase Transgen Cat #: AP221, Lot#: N30507 dNTPs Novoprote...
Identified variants were validated by Sanger sequencing. PCR amplification was performed using the TransStart FastPfu FLY DNA Polymerase (AP231, civic bioscience) following instructions of the manufacturer with primers described in supplemental Table4. Sanger sequencing of amplicons was performed on an ABI...