Subsequently, the slides or cells were blocked with 5% normal goat serum (NGS) and 0.1% Tween-20 in tris buffered saline (TBS) for 30 min at room temperature. The samples were then incubated with primary antibodies overnight at 4 °C. After washing thrice with TBS, the cells were ...
Serum Treatment: Standard (Sterile-Filtered) Country of Origin: New Zealand Green Features: Sustainable packaging Shipping Condition: Dry Ice 内容及储存 Storage conditions: -5 to -20°C Shipping conditions: Frozen目录号:16210064 品牌:ThermoFisher /Invitrogen/ Gibco/ ABI 规格:100ML 目录价:￥629.00 ...
For the immunofluorescence analysis of α6, frozen sections from healthy donors and patients were fixed with 2% paraformaldehyde in phosphate-buffered saline, washed in phosphate-buffered saline and permeabilized with 0.15% Triton X-100, saturated with 5% normal goat serum (Sigma, St Louis, MO) ...
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For flow cytometry analysis, cells were fixed in 2% paraformaldehyde in PBS for 20 min at 4 °C and sequentially blocked with blocking buffer (PBS containing 5% goat normal serum and 1% BSA) for 1 h at 4 °C. Cells were stained with the appropriate first antibody (5 µg/...
Solution B(Normal Goat Serum For Blocking,White Solution) 3ml Solution C(Goat anti-Rabbit IgG,Biotin,Ready to Use,Yellow Solution) 3ml Solution D(Streptavidin-HRP,Ready to Use,Red Solution) 3ml DAB-A(20×) 250μl DAB-B(1×) 5ml注意事项:1、本试剂盒仅适用于一抗为兔源抗体的IHC实验。2、...
receptors, SCARA5 does not endocytose acetylated or oxidized low density lipoprotein. Instead, SCARA5 binds serum ferritin and mediates its endocytosis from the cell surface, indicating SCARA5 may play a role in non-transferrin iron delivery. At least four isoforms of SCARB1 are known to exist....
注意事项:闹羊花毒素III26342-66-5说明书纯度高,检测/鉴别精确,稳定性强,为了防止产品含量降低,不宜暴露在空气中,应防潮、避光和抗氧化包装,保存应以提高物质稳定性为原则,尽可能在干燥、低温条件下储藏(液体或易潮解物质分装在熔封的玻璃安瓶内)。 产品名称:闹羊花毒素III26342-66-5说明书 ...
NS02L-1ML Normal Goat Serum 1PC X 1ML NS03L-1ML Normal Mouse Serum NS1004 OligodendrocyteProgenitorCell IsolatKit NS1226-1MG CysteineSulfenicAcidProbe(DCP-Bio-1)-1MG NS1226-5MG CysteineSulfenicAcidProbe(DCP-Bio-1)-5MG NS145FR DOPAMINERGIC NEURON INTEG KIT (-20) NS145RF DOPAMINERGIC NEURON...
The cells were blocked with 10% goat serum and incubated with the primary antibody at 5 µg/mL. Cy3 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:... View More 产品信息 应用 建议稀释比 已发表文章 免疫印迹 (WB) 0.1-0.5 µg/mL - 免疫组化(石蜡) (IHC ...