293 whole cell lysate, Lane 2: A431 whole cell lysate, Lane 3: mouse brain lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1:10,000 dilution. Predicted band size : 45 kDa. Blocking/Dilution buffer: 5% NFDM/TBST...