【解析】RACE(rapid amplification of cDNA ends)技术是用于从已知cDNA片段扩增全长基因的方法, 它根据已知序列设计基因片段内部特异引物,由该片段向外侧进行PCR扩增得到目的序列。用于扩增$$ 5 $$ 端的方法称为5'RACE,用于扩增 $$ 3 ^ { \prime } $$端的称为$$ 3 ^ { \prime } R A C E 。...
LONDON, Feb. 24 (Xinhua) -- China's telecommunications giant Huawei, which has been enhancing cooperation with big telecom operators across Europe and Asia, is on its way to occupying "a prime position" to lead the next-generation 5G networks global race, Reuters reported. Huawei has been co...
百度试题 结果1 题目(B)5. $$ \fr a c { L c t ^ { \prime } s } { A } $$to have a race have 相关知识点: 试题来源: 解析 答案见上 反馈 收藏
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【答案】(1)5'RACE ①在反转录胞的作用下,以已知基因片段内部特异性引物起始CDNA第一条链的合成。 ②RNase混合物降解模板mRNA,纯化cDNA第一条链。 ③用末端转移酶在cDNA链 $$ 3 ^ { \circ } $$端加入连线的 $$ d C T P $$,形成oligo dC尾巴。 ④以连有 oligo dC的错定引物和基因片段内部特异...