Alonso JM, Garrido-Pertierra A (1987) Carboxymethylhydroxymuconic semialdehyde dehydrogenase in the 4-hydroxyphenylacetate catabolic pathway of Pseudomonas putida . Biochem Cell Biol 64:1288–1293Alonso, J. M., and A. Garrido-Pertierra. 1986. Carboxymethylhydroxymu- conic semialdehyde dehydrogenase in ...
α-Hydroxy-γ-carboxymuconic -semialdehyde (HCMS) dehydrogenase was purified about 180-fold with a yield of 22% from a cell-free extract of . The enzyme was adsorbed tightly on blue dextran, possibly at its coenzyme binding sites. This finding made possible a simple purification step by molecu...