4-Hydroxybenzoate-polyprenyltransferase, an enzyme in ubiquinone biosynthesis, from Pseudomonas putida was partially purified by ion-exchange and gel filtration column chromatography. The enzyme required phosph
The enzymes are apparently quite specific for the chain length of the isoprenoid precursors, which is in contrast to the broad substrate specificity of the polyprenyldiphosphate: 4-HB polyprenyltransferase from Escherichia coli.doi:10.1016/S0031-9422(96)00458-XRobert Boehm...
PHB:polyprenyltransferasedoi:10.1016/S0021-9258(19)45124-7Kazutaka MomoseHarry RudneyJournal of Biological ChemistryK. Momose, H. Rudney, 3-Polyprenyl-4-hydroxybenzoate synthesis in the inner membrane of mitochondria from p-hydroxybenzoate and isopentenylpyrophosphate. A demonstration of isoprenoid synthesis...
The ubiA and CoQ2 genes, which are involved in the synthesis of 4-hydroxybenzoate polyprenyltransferase, were cloned and overexpressed in A. cinnamomea. The results showed that ubiA and CoQ2 overexpression significantly increased AQ production in submerged fermentation. The overexpressing strain produced...