A cDNA clone encoding 4-aminobenzoate hydroxylase (EC 1.14.13.27) has been isolated using a probe prepared by PCR on the basis of partially determined amino acid sequences of the enzyme. The cDNA contained 1380-base pair open reading frame encoding 460 amino acid residues (Mr 50 974), 14-...
StereospecificityNAD(P)H oxidation4-Aminobenzoate hydroxylase(A. bisorus)The stereospecificity of NAD(P)H oxidation catalyzed by 4-aminobenzoate hydroxylase, a new FAD-dependent monooxygenase from Agaricus bisporus, was examine. Isotropic studies with [4A- 3H]- and [4B- 3H]NAD(P)H showed that ...
Determines the crystal structures of wild-type p-hydroxybenzoate hydroxylase from Pseudonyms fluoresces, complexed with the substrate analogs 4-aminobenzoate, 2,4-dihydroxybenzoate, and 2-hydroxy-4-aminobenzoate at 2.3-, 2.5-, and 2.8 A resolution. Description of experimental procedure; Results of ...
A new assay method of γ-glutamyltransferase with 4-aminobenzoate hydroxylase from Agaricus bisporus as a coupling enzyme[J] . Yukihito Mizutani,Yorito Nakano,Shingo Yamada,Tatsuya Samejima.Clinica Chimica Acta . 1999 (1)A new assay method of γ-glutamyltransferase with 4-aminobenzoate hydroxylase ...
Four monoclonal antibodies (mAbs) against 4-aminobenzoate hydroxylase (EC 1.14.13.27) have been produced (H. Tsuji et al., J. Biol. Chem. 265 (1990) 16064; T. Ogawa et al., Biochim. Biophys. Acta 1115 (1992) 220). Of the mAbs, three mAbs (mAb-A, -B1 and -B2) recognize the ...