Preparation Here we are describing steps for 4% PFA of the 1L solution. The same trend follows for other volumes as well. Take 800 mL of 1X PBS. Add 40 g of Paraformaldehyde powder to 1X PBS. Stir the mixture at 60˚C in ventilation hood (DO NOT Boil). PFA powder does not diss...
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Product Name4% tissue cell fixative (4% PFA, RNASE FREE) is paraformaldehyde solution (4% PFA, RNASE FREE) CAS CBNumberCB312870131 MW0 MOL FileMol filePreparation Products And Raw materials4% tissue cell fixative (4% PFA, RNASE FREE) is paraformaldehyde solution (4% PFA, RNASE FREE) ...
Briefly, cells were first stained with 500 nM MitoTracker Deep Red (Thermo Fisher) in 2 ml culture medium and incubated at 37 °C in 5% CO2 for 30 min, followed by fixation in 4% paraformaldehyde (PFA) dissolved in PBS. Fixed cells were then permeabilized in 0.2% Triton X-...
C2C12 myoblasts (1 × 107) were harvested and treated with 1% paraformaldehyde (PFA) to cross-link proteins to DNA. The cells were lysed in the presence of protease inhibitors (Roche), sonicated to shear DNA into fragments, and incubated with an antibody against FOXO1 (1:50, 2880; Cel...
Hans R. PfaendlerUSUS4683303 * 1984年10月30日 1987年7月28日 Pfaendler Hans R Reduction process for the preparation of 4-unsubstituted azetidin-2-onesUS4683303 * 1984年10月30日 1987年7月28日 Pfaendler; Hans R. Reduction process for the preparation of 4-unsubstituted azetidin-2-ones...
PREPARATION:The compound of formula I can be produced by the cyclization reaction of the compound of formula II. The reaction is carried out in an organic solvent such as benzene, toluene, etc. in the presence of a condensation agent such as N,N'-dicyclohexyl-carbodiimide, diethyl ...
Synthesis of a Phospfaoramidite Coupling Unit of the Pyrimidine (6—4) Pyrimicione Photoproduet and Its Incorporation into OligodeoxynucleotidesUltraviolet (UV) light causes lesions in DNA, which induce mutations, cellular transformation, and cell death. At adjacent pyrimidme sites, two major ...
Samples were post-fixed in 2% PFA for 10 min, quenched for 5 min, rinsed and mounted for imaging. Calibration and alignment markers Before mounting the sample, fluorescent beads were added on the surface of the coverslip, which act as fiducial markers for registration of the 4Pi image ...
40. Chromosomal tagging of theATG1orATG4genes at the 3’end was done by PCR-based integration of the N-terminus or the C-terminus of the Venus tag using pFA6a-VN-His3MX6 and pFA6a-VC-TRP1 as template plasmids, respectively25. PCR verification, western blotting using antibodies ...