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After fixation, cells were washed three times in PBS and then extracted for 1 min in Karsenti's solution (80 mM Pipes, 5 mM EGTA, 1 mM MgSO4 and 0.5% Triton X-100). Cells were then post-fixed in 1 mg ml À 1 NaBH4 for 10 min, and then rehydrated in PBS þ 0.1% Tween ...
(e) Confocal images illustrating the different intensities of Orai1 and Orai2 immunofluorescence in control cells (upper panel) and cells exposed to LPS (middle panel) or poly(I:C) (lower panel). (f) Representative western blot of Orai2 in control cells and cells exposed to LPS or poly...
The Au(111) crystal (MaTecK, Jülich, Germany, 4 mm diameter, 0.126 cm2 surface area) was flame-annealed before dipping for 10 min into a deaerated 20 µM solution of 4,4′-dithiodipyridine (Acros, 98%) in ultrapure water (18.2 MΩ cm, < 5 pbb TOC). During the adsorption ...
CIPOS™ Micro 600 V, 4 A three-phase RCD2 IGBT based intelligent power module with open emitter in SOP 29x12 package providing fully-featured compact inverter solution for low motor drive applications like fans, pumps and light load appliances.
Magnesium gallate (MgGa 2 O 4 ) was synthesized by solution combustion synthesis method at 500°C. The X-ray diffraction (XRD) pattern indicates that the prepared compound crystalized into a single phase, and a porous morphology associated with combustion was observed from the image of the ...
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The peptide mix was desalted using C18 ZipTips (Millipore, Burlington, MA, USA) and 1 µL of this solution was combined with 1 µL of a 3 mg−1mL α-cyano-4-hydroxycinnamic acid (60% acetonitrile, 1 mM ammonium diphosphate) and spotted onto MALDI targets. All MALDI-MS ...