nutrients Article Spirulina maxima Extract Reduces Obesity through Suppression of Adipogenesis and Activation of Browning in 3T3-L1 Cells and High-Fat Diet-Induced Obese Mice Young-Jin Seo †, Kui-Jin Kim †, Jia Choi, Eun-Jeong Koh and Boo-Yong Lee * Department of Food Science and ...
2.5. Oil-Red O Staining Intracellular lipid accumulation was determined using Oil-Red O staining (Thermo Fisher Scientific). Differentiated adipocytes (day 11) were rinsed with phosphate-buffered saline (PBS) twice and were fixed in 10% buffered formalin for 1 h at room temperature. After ...